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. 2017 Sep 15;10:288. doi: 10.3389/fnmol.2017.00288

Figure 2.

Figure 2

Effects of serum and mTOR inhibition by rapamycin on serum-induced mTOR activity, CRMP-2 and synapsin-I expression and neuronal proliferation in HT-22 cells. Cells were cultured for 24 and 48 h in serum-containing media, and viable cell levels were compared using a CCK-8 (A), and the p-S6K/S6K and CRMP-2 levels were analyzed by Western blotting, and subsequently quantified (B–D). Cells in serum-free media were switched to culture in serum containing media in the absence or presence of rapamycin (5 μM) for 24 h, and viable cell levels were assayed (E) and p-mTOR/mTOR, p-S6K/S6K, p-S6/S6, CRMP-2 and synapsin-I were analyzed by Western blotting (F). The quantitative data are the mean ± SEM of five experiments. Significance values indicate *p < 0.05 and **p < 0.01 vs. 0 h + serum control (A,C,D) or vs. 24 h − serum control; ††p < 0.01 vs. 24 h + serum control.