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. 2017 Sep 15;8:1777. doi: 10.3389/fmicb.2017.01777

Figure 2.

Figure 2

(A) Physical maps of YRC102-AYNI1-AGDC1-6H, YIC102-AYNI1-AGDC1-6H transformed into A. adeninivorans G1212. Both cassettes contained the selection marker module with ATRP1m gene fused to the ALEU2 promoter and the expression module with AYNI1 promoter—AGDC1-6H gene—PHO5 terminator. In addition, YRC (AscI fragment) is flanked by 25S rDNA sequences for targeting the cassette into genomic 25S rDNA. YIC (Sbf I fragment) contain the selection marker and expression module and was integrated randomly into the genome. (B) Time-course traces of A. adeninivorans G1212/YRC102 and (C) G1212/YRC102-AYNI1-AGDC1-6H grown on YMM-glucose-NaNO3 in shake flasks at 30°C for 146 h. Intracellular Agdc1-6hp activity (triangles) detected with gallic acid as substrate, calculated yield [Y(P/X)] (circles) and dry cell weight (squares). Measurements were done in triplicate.