Fig. 3.

Ribo-seq of neurites and soma demonstrates that neurite-enriched proteins are locally translated. a Schematic representation of local Ribo-seq. b Correlation heatmap of individual Ribo-seq libraries, prepared from neurites and soma of iNeurons (three biological replicates). c Ribo-seq reads show subcodon resolution supported by a strong bias toward the translated frame (frame 0) and 3 nt periodicity. Read length: 29 nt. d Local Ribo-seq from neurites and soma. Enrichment of Ribo-seq reads in neurite versus soma plotted against average abundance of Ribo-seq reads (RPKM mapped to CDS). Green: transcripts preferentially translated in neurites (neurites/soma log2FC > 1, P-values < 0.05). e RNA enrichment in neurites plotted against protein enrichment as in Fig. 2d, and color-coded for enrichment of Ribo-seq reads in neurites. Green: genes preferentially translated in neurites according the Ribo-seq data