FIGURE 3.

Effect of tacrolimus, itraconazole, and fluconazole – as monotherapy, or in synergistic combinations – on the levels of neutral lipids in Sporothrix brasiliensis CBS 133021 (A) and S. schenckii CBS 132984 (B). Yeast cells were treated with drugs for 48 h, stained with BODIPY 493/503 and then analyzed by flow cytometry. The following drug concentrations were used: 0.5 mg/L tacrolimus, 0.01 mg/L itraconazole, and 1 mg/L fluconazole. The combination of tacrolimus with itraconazole increased considerably the levels of neutral lipids in S. brasiliensis yeast cells, relative to the untreated control. Data represent mean ± SEM values of three independent experiments. ^∗p < 0.05, ^∗∗∗p < 0.001 vs. untreated (by one-way ANOVA with Dunnett’s test).