FIGURE 1.

Body weight changes and survival rates of mice inoculated with mouse-adapted H5N6 viruses. Groups of six 6-week-old BALB/c female mice were inoculated i.n. with 50 μL of P10, P1, and WT H5N6 viruses 10⁶ EID₅₀/mL of or PBS. Body weight changes and survival rates were daily monitored for 14 days. The mouse body weights were represented as the average weight percentage on the day of inoculation (day 0). The average body weights of each group were shown and error bars represented the standard deviation (SD). (A) Percent change in body weight from day 0. Body weight data was analyzed using two-way ANOVA method and the comparisons between two groups were made using SNK method. ^∗P < 0.05; ^∗∗P < 0.01. The blue asterisks represent the significant difference between P1 and WT, while the green asterisks represent the significant difference between P10 and WT. (B) Survival rates. Survival data comparisons between each two groups (P10 vs WT, P1 vs WT, and P10 vs P1) were analyzed with GraphPad Prism 5.0 software using the log-rank test method, and the P-values are indicated in the graph.