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. 2017 Sep 19;7:11900. doi: 10.1038/s41598-017-11630-1

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Mutation of Hdac11 gene results in loss of HDAC11 expression. (A) A primer pair (arrows) requiring a sequence in the deleted region (dotted box) encompassing exon 3 of the Hdac11 gene fails to produce a 284 bp in Hdac11 ^KO/KO (HOM) mice. (B) A primer pair that covers the deleted region yields a smaller product in mice heterozygous or homozygous for the mutant allele. (C,D) Lower levels of the Hdac11 transcript and (F) HDAC11 protein were detected in Hdac11 ^WT/KO mice compared to Hdac11 ^WT/WT mice. Gene expression was normalised to that of Gapdh. (E) No HDAC11 protein was detected in Hdac11 ^KO/KO mice. (G) A truncated form of HDAC11 was not detected in Hdac11 ^KO/KO mouse brain. Antibody used in panels 4E, 4F and 4G is anti-HDAC11 (Sigma, Catalogue #H4539). N = 6 (dots = individual sample values), bars = mean ± STD, **p < 0.01, one-tailed unpaired Student’s t-test.