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. 2017 Sep 19;7:11900. doi: 10.1038/s41598-017-11630-1

Figure 6.

Figure 6

Reduced Fez1 expression in neural cells derived from Hdac11 KO/KO mice. (A) Three neural cell lines were generated from Hdac11 WT/WT, Hdac11 WT/KO and Hdac11 KO/KO mice. (B) Cells were counted following each passage every 4 days for 10 passages. (C) Cells derived from all mice exited the cell cycle following 3 days of differentiation inducing conditions. (D) Presence of EdU, Nestin (NES), TUBB3, and GFAP in cells derived from the ganglionic eminence of E14.5 Hdac11 KO/KO mouse embryos following 3 days of proliferation or differentiation inducing conditions. (E) Relative expression of genes associated with neural proliferation in mouse embryo-derived neural cells given 3 days of either proliferation (PRO) or differentiation (DIF) associated conditions. Msi2 and Olig2 expression display opposing changes during the differentiation of Hdac11 WT/WT and Hdac11 KO/KO cells (arrow heads). The expression of Fez1 (arrow) was decreased in neural cells derived from Hdac11 KO/KO mice following 3 days of either (F) proliferating or (G) differentiating inducing conditions. (H) Fez1 expression increased in neural cells derived from Hdac11 WT/WT and Hdac11 KO/KO mice at similar levels following 3 days differentiation. N = 3 (dots = individual sample values), bars = mean ± SE, **p < 0.01, *p < 0.05, two-tailed unpaired Student’s t-test. Gene expression was normalised to the mean of Canx and Sdha.