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. 2017 Sep 19;7:11857. doi: 10.1038/s41598-017-12072-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Intracellular scavenging of tBHP-induced radicals by antioxidants. (a) Intracellular ROS production in SH-SY5Y cells treated with tBHP in the absence or presence of Proxison (10 μM), quercetin (100 μM), or myricetin (100 μM) was observed with CellROX® Deep Red. Scale bar: 40 μm. (b) The average intensity of CellROX® Deep Red (far red) fluorescence of cells in the five conditions were quantified with Volocity software. Bars represent the mean from four independent experiments with duplicate cover slips for Proxison, and two independent experiments for quercetin and myricetin. Error bars represent the SEM. ANOVA with the post-hoc Tukey test was performed to assess significant difference to vehicle-treated cells; **p < 0.01, ***p < 0.001.