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. 2017 Sep 19;7:11857. doi: 10.1038/s41598-017-12072-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Antioxidant protection against 6-OHDA-induced dopaminergic cell death in zebrafish embryos. (a) Zebrafish embryos at 24 hours post-fertilisation (hpf) were treated with 6-OHDA for 24 hours in the presence or absence of the antioxidants Proxison or quercetin (10 μM). Embryos were then fixed at 48 hpf, immunostained for TH (red) and confocal images were taken of the embryo head viewed from the top. Scale bar: 50 μm. (b) The number of TH-positive cells in each embryo was counted for each treatment group (control, n = 28; vehicle, n = 30; Proxison, n = 26, quercetin, n = 24). Bars represent the mean number of TH-positive cells and error bars show the SEM. ANOVA with Tukey’s post-hoc test was performed to assess the significant difference to the 6-OHDA treated embryos; *p < 0.05, ***p < 0.001.