Fig. 5.

Caspase-dependent pruning of class IV ddaC dendrites during metamorphosis requires tango7 but not dark. a, b Dendritic arborizations, visualized by ppk-GAL4-driven expression of GFP, are present at −8 h PF a, but are completely pruned by + 16 h PF b. c, d Expression of a dominant-negative ecdysone receptor (EcR ^F645A) using ppk-GAL4 (c) or mutation of dronc (d) disrupts pruning at + 16 h PF. e, f Fewer dendritic arborizations form at −8 h PF upon RNAi knockdown of tango7 (using ppk-GAL4) e; however, these branches are not properly pruned at + 16 h PF f. g, h Dendritic pruning occurs normally in dark (g) or drice (h) mutant animals. Red arrowheads indicate the soma of ddaC neurons. Scale bars represent 100 µm. Df, deficiency, PF, puparium formation