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. 2017 Sep 19;7:11888. doi: 10.1038/s41598-017-12244-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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HPLC traces of APPT reactions with 11 CP substrates. Red traces represented the enzyme reactions and black ones showed the substrates. ▼ indicated the holo-CP, while ∇ represented the apo-substrate. (A) SFACP, AFACP and SsPCP were partially phosphopantetheinylated over the course of overexpression in E. coli, and were fully converted in the enzyme reactions. (B) All other CPs were in apo-forms and completely functionalized in the APPT reactions. Similar traces were observed in the reactions of several other PPTases.