Table 1.

Kinetics parameters of four selected PPTases toward 11 CPs^a.

Substrate	APPT			MPPT			SPPT			Sfp
	K m b	k cat b	k cat/K m b	K m	k cat	k cat/K m	K m	k cat	k cat/K m	K m	k cat	k cat/K m
SFACP	2.8 ± 0.2	1.6 ± 0.09	0.6 ± 0.07	3.2 ± 0.2	1.2 ± 0.1	0.4 ± 0.07	1.5 ± 0.2	1.4 ± 0.1	0.9 ± 0.2	2.5 ± 0.3	2.2 ± 0.1	0.9 ± 0.2
AFACP	6.8 ± 0.5	11.7 ± 0.5	1.7 ± 0.2	6.9 ± 0.4	8.4 ± 0.2	1.2 ± 0.1	13.3 ± 1.6	14.7 ± 1.1	1.1 ± 0.2	11.6 ± 0.6	9.8 ± 0.3	0.8 ± 0.07
APACP	10.0 ± 0.9	17.0 ± 0.6	1.6 ± 0.2	23.1 ± 4.1	21.2 ± 1.6	0.9 ± 0.2	26.5 ± 5.2	14.6 ± 1.3	0.5 ± 0.1	15.3 ± 2.1	22.1 ± 1.4	1.5 ± 0.3
ScACP	14.1 ± 1.6	5.4 ± 0.3	0.4 ± 0.07	12.4 ± 1.5	2.6 ± 0.1	0.2 ± 0.04	N/Ac	N/Ac	N/Ac	8.3 ± 0.5	15.2 ± 0.5	1.8 ± 0.1
SsPCP	7.4 ± 0.5	7.6 ± 0.2	1.0 ± 0.09	9.1 ± 0.3	7.1 ± 0.1	0.7 ± 0.04	14.3 ± 1.1	1.0 ± 0.04	0.06 ± 0.008	7.9 ± 0.7	16.7 ± 0.7	2.1 ± 0.2
FNPCP	12.1 ± 0.5	12.2 ± 0.3	1.0 ± 0.07	7.2 ± 0.7	9.6 ± 0.4	1.3 ± 0.2	7.0 ± 0.6	10.1 ± 0.4	1.4 ± 0.2	11.7 ± 0.8	14.5 ± 0.6	1.2 ± 0.1
FisPCP	7.1 ± 0.5	2.3 ± 0.06	0.3 ± 0.03	7.2 ± 0.3	1.9 ± 0.03	0.3 ± 0.02	10.0 ± 0.5	2.5 ± 0.05	0.3 ± 0.02	14.0 ± 1.2	1.7 ± 0.07	0.1 ± 0.02
MACP	7.9 ± 0.9	3.7 ± 0.2	0.5 ± 0.08	4.9 ± 0.3	4.2 ± 0.1	0.9 ± 0.07	22.0 ± 1.6	4.3 ± 1.1	0.2 ± 0.06	6.7 ± 0.7	5.1 ± 0.2	0.8 ± 0.1
APNPCP	1.6 ± 0.1	1.0 ± 0.05	0.6 ± 0.09	9.5 ± 0.4	1.9 ± 0.1	0.2 ± 0.02	17.0 ± 1.3	2.3 ± 0.2	0.1 ± 0.02	12.0 ± 0.9	2.2 ± 0.2	0.2 ± 0.02
FNsACP	8.4 ± 0.8	2.2 ± 0.08	0.3 ± 0.03	8.3 ± 0.8	2.1 ± 0.4	0.3 ± 0.07	14.1 ± 1.4	1.5 ± 0.07	0.1 ± 0.02	10.9 ± 0.5	2.4 ± 0.05	0.2 ± 0.01
AprACP	7.8 ± 0.8	14.3 ± 0.8	1.8 ± 0.3	9.1 ± 1.4	10.4 ± 0.8	1.1 ± 0.3	12.9 ± 2.1	13.1 ± 1.2	1.0 ± 0.2	9.0 ± 1.0	2.9 ± 0.1	0.3 ± 0.05

^aThe data represented mean ± SD of three independent experiments; ^bUnits of K _m, k _cat, and k _cat/K _m are µM, min⁻¹, and µM⁻¹ min⁻¹, respectively; ^cNo detectable activity.