Figure 6.

T cell receptor (TCR)-γδ⁺ T-cell proportions are reduced in pregnancy but they express greater progesterone-induced blocking factor (PIBF). These changes are primarily seen in the second trimester. (A) Shows changes in proportions of TCR-γδ CD4 and CD8 T cells in response to progesterone culture. (B) Alterations in PIBF expression on the aforementioned TCR-γδ T cells. (C) Peripheral blood mononuclear cell (PBMCs) were surface stained with anti-CD3, anti-CD8, anti-TCR-γδ, and anti-PIBF. Gating strategy used to phenotype CD4⁺/CD8⁺ TCR-γδ⁺ PIBF⁺ T-cells. Box and whiskers plots indicate median, interquartile range and 10th–90th centiles. ^♯p < 0.05 and ^♯♯p < 0.01 (Kruskal–Wallis one way analysis of variance and Dunn’s test for multiple comparisons). Abbreviations: HC, healthy control; Pr, pregnant.