Table 1.
List of antibodies used for RPPA analysis
| Antibody name | Company | Catalog # | Host |
|---|---|---|---|
| 14-3-3zeta,gamma,eta_R_V | EMD Millipore (Upstate) | 06-408 | Rabbit |
| Akt_R_V | CST | 9272 | Rabbit |
| p-Akt(S473)_R_V | CST | 9271 | Rabbit |
| p-Akt(T308)_R_V | CST | 9275 | Rabbit |
| p-ALK(3B4)(Y1586)_R_V | CST | 3348 | Rabbit |
| p-ALK(Y1604)_R_V | CST | 3341 | Rabbit |
| p-AMPKa(40H9)(T172)_R_V | CST | 2535 | Rabbit |
| p-AMPKa1(S485)_R_V | CST | 4184 | Rabbit |
| p-AMPKb1(S108)_R_V | CST | 4181 | Rabbit |
| Annexin1_R_V | Invitrogen | 71-3400 | Rabbit |
| ATM_R_V | Abcam | ab32420 | Rabbit |
| ATR_R_V | CST | 2790 | Rabbit |
| p-ATR(S428)_R_V | CST | 2853 | Rabbit |
| p-AuroraA(T288)/B(T232)/C(T198)_R_V | CST | 2914 | Rabbit |
| AuroraA/AIK_R_V | CST | 3092 | Rabbit |
| p-Axl(D12B2)(Y702)_R_V | CST | 5724 | Rabbit |
| Bad_R_V | CST | 9292 | Rabbit |
| p-Bad(S112)_R_V | CST | 9291 | Rabbit |
| p-Bad(S136)_R_V | CST | 9295 | Rabbit |
| p-Bad(S155)_R_V | CST | 9297 | Rabbit |
| Bak_R_V | CST | 3814 | Rabbit |
| Bax_R_V | CST | 2772 | Rabbit |
| p-Bcl2(5H2)(S70)_R_V | CST | 2827 | Rabbit |
| p-Bcl2(T56)_R_V | CST | 2875 | Rabbit |
| BRCA1_R_V | EMD Millipore (Upstate) | 07-434 | Rabbit |
| BRCA2_R_V | CST | 9012 | Rabbit |
| Caspase-3_R_V | CST | 9662 | Rabbit |
| p-Beta-Catenin(S33/37/T41)_R_V | CST | 9561 | Rabbit |
| Caveolin-1(D46G3)XP_R_V | CST | 3267 | Rabbit |
| p-Caveolin-1(EPR2288Y)(Y14)_R_V | Abcam-Epitomics | 2267-1 | Rabbit |
| CD24(FL-80)_R_V | Santa Cruz Biotechnology | sc-11406 | Rabbit |
| p-Chk1(S345)_R_V | CST | 2341 | Rabbit |
| p-Chk2(S33/35)_R_V | CST | 2665 | Rabbit |
| Claudin-1_R_V | CST | 4933 | Rabbit |
| c-Met_R_V | Abcam | ab51067 | Rabbit |
| p-c-Myc(T58)_R_V | Abcam | ab28842 | Rabbit |
| DKK1_R_V | CST | 4687 | Rabbit |
| EGFR(D38B1)XP_R_V | CST | 4267 | Rabbit |
| EGFR(L858R)_R_V | CST | 3197 | Rabbit |
| p-EGFR(S1046/1047)_R_V | CST | 2238 | Rabbit |
| p-EGFR(Y1045)_R_V | CST | 2237 | Rabbit |
| p-EGFR(Y1148)_R_V | CST | 4404 | Rabbit |
| p-EGFR(Y1173)_R_V | Invitrogen (BioSource) | 44-794G | Rabbit |
| p-EGFR(Y1173)(53A5)_R_V | CST | 4407 | Rabbit |
| p-EGFR(Y1068)(D7A5)XP_R_V | CST | 3777 | Rabbit |
| Ezh2(D2C9)XP_R_V | CST | 5246 | Rabbit |
| FGFR1(D8E4)XP_R_V | CST | 9740 | Rabbit |
| FoxO1(C29H4)_R_V | CST | 2880 | Rabbit |
| FSP1/S100A4_R_V | EMD Millipore | Jul-74 | Rabbit |
| p-HER2/ErbB2(Y1248)_R_V | CST | 2247 | Rabbit |
| p-HER2/ErbB2(Y877)_R_V | Imgenex | IMG-90185 | Rabbit |
| HER3/ErbB3_R_V | CST | 4754 | Rabbit |
| p-HER3/ErbB3(Y1197)_R_V | CST | 4561 | Rabbit |
| HDAC1_R_V | CST | 2062 | Rabbit |
| HDAC3_R_V | CST | 2632 | Rabbit |
| HDAC4_R_V | CST | 2072 | Rabbit |
| HDAC6_R_V | Santa Cruz Biotechnology | sc-11420 | Rabbit |
| ILK1_R_V | CST | 3862 | Rabbit |
| p-Jak1(Y1022/1023)_R_V | CST | 3331 | Rabbit |
| Kit-c_R_V | Abcam | ab32363 | Rabbit |
| Lipocalin-1_R_V | Santa Cruz Biotechnology | sc-66943 | Rabbit |
| LRP6_R_V | CST | 2560 | Rabbit |
| MEK1_R_V | Abcam-Epitomics | 1235-1 | Rabbit |
| p-MEK1/2(S217/221)_R_V | CST | 9121 | Rabbit |
| p-Met(Y1234/1235)_R_V | CST | 3077 | Rabbit |
| MMP-9_R_V | CST | 3852 | Rabbit |
| mTOR_R_V | CST | 2972 | Rabbit |
| p-mTOR(D9C2)XP(S2448)_R_V | CST | 5536 | Rabbit |
| Notch1(C44H11)_R_V | CST | 3268 | Rabbit |
| p-p27(T187)_R_V | Invitrogen (Zymed) | 71-7700 | Rabbit |
| p-p27/KIP1(T198)_R_V | Abcam | ab64949 | Rabbit |
| p27/KIP1(C-term)_R_V | Abcam-Epitomics | ab32034(1591-1) | Rabbit |
| p38/MAPK_R_V | CST | 9212 | Rabbit |
| p-p38(D3F9) XP(T180/Y182)_R_V | CST | 4511 | Rabbit |
| p44/42MAPK(Erk1/2)_R_V | CST | 9102 | Rabbit |
| p-p44/42MAPK(Erk1/2)(T202/Y204)(197G2)_R_V | CST | 4377 | Rabbit |
| p53_R_V | CST | 9282 | Rabbit |
| p-p53(S15)_R_V | CST | 9284 | Rabbit |
| p70S6K_R_V | CST | 9202 | Rabbit |
| p-p70S6K(S371)_R_V | CST | 9208 | Rabbit |
| p-p70S6K(T389)_R_V | CST | 9205 | Rabbit |
| p-p70S6K(T412)_R_V | EMD Millipore (Upstate) | 07-018 | Rabbit |
| p-PDGFRa(23B2)(Y754)_R_V | CST | 2992 | Rabbit |
| PDGFRb(28E1)_R_V | CST | 3169 | Rabbit |
| p-PDK1(S241)_R_V | CST | 3061 | Rabbit |
| PIAS1_R_V | CST | 3550 | Rabbit |
| PTEN(D4.3)XP_R_V | CST | 9188 | Rabbit |
| p-PTEN(S380)_R_V | CST | 9551 | Rabbit |
| p-RafB(S445)_R_V | CST | 2696 | Rabbit |
| RANKL_R_V | Novus | NBP1-31140 | Rabbit |
| p-Ret(Y905)_R_V | CST | 3221 | Rabbit |
| Slug(C19G7)_R_V | CST | 9585 | Rabbit |
| p-Smad2(S465/467)_R_V | CST | 3101 | Rabbit |
| SOCS1(A156)_R_V | CST | 3950 | Rabbit |
| SOCS3_R_V | CST | 2923 | Rabbit |
| Sox2(D6D9) XP_R_V | CST | 3579 | Rabbit |
| p-Src(Y527)_R_V | CST | 2105 | Rabbit |
| Stat1_R_V | CST | 9172 | Rabbit |
| p-Stat1(Y701)_R_V | CST | 9171 | Rabbit |
| p-Stat2(Y690)_R_V | CST | 4441 | Rabbit |
| p-Stat3(S727)_R_V | CST | 9134 | Rabbit |
| p-Stat4(Y693)_R_V | CST | 5267 | Rabbit |
| p-Stat5(Y694)_R_V | CST | 9351 | Rabbit |
| Stat6_R_V | CST | 9362 | Rabbit |
| p-Stat6(Y641)_R_V | CST | 9361 | Rabbit |
| p-Tuberin/TSC2(T1462)_R_V | CST | 3611 | Rabbit |
| Vimentin(D21H3)XP_R_V | CST | 5741 | Rabbit |
| Wnt5a/b(C27E8)_R_V | CST | 2530 | Rabbit |
| YAP(H125)_R_V | Santa Cruz Biotechnology | sc-15407 | Rabbit |
| ZO-1_R_V | CST | 5406 | Rabbit |
| Caspase-7_R_V | CST | 9491 | Rabbit |
| VEGFR2(55B11)_R_V | CST | 2479 | Rabbit |
| Stat5a(L-20)_R_V | Santa Cruz Biotechnology | sc-1081 | Rabbit |
| SOX9_R_V | EMD Millipore (Upstate) | AB5535 | Rabbit |
| p-SAPK/JNK(T183/Y185)_R_V | CST | 4668 | Rabbit |
| Zeb1_R_V | Novus | NBP1-05987 | Rabbit |
| PHF8(pAb)_R_V | Active Motif | 39711 | Rabbit |
| ASH2_R_V | Bethyl Laboratories | A300-489A | Rabbit |
| CBP(A-22)_R_V | Santa Cruz Biotechnology | sc-369 | Rabbit |
| c-Fos(9F6) _R_V | CST | 2250 | Rabbit |
| CHAF1A(D77D5)XP_R_V | CST | 5480 | Rabbit |
| c-Jun(60A8)_R_V | CST | 9165 | Rabbit |
| CRSP1-TRAP220_R_V | Bethyl Laboratories | A300-793A | Rabbit |
| CtBP2_R_V | CST | 13256 | Rabbit |
| Cyclin C_R_V | Santa Cruz Biotechnology | sc-1061 | Rabbit |
| DRIP130_R_V | Abcam | ab70450 | Rabbit |
| FBX011_R_V | Abcam | ab72200 | Rabbit |
| FoxK2_R_V | CST | 12008 | Rabbit |
| KLF4_R_V | CST | 12173 | Rabbit |
| MED12-Abcam_R_V | Abcam | ab70842 | Rabbit |
| p300(C-20)_R_V | Santa Cruz Biotechnology | sc-585 | Rabbit |
| p-c-Fos(S32)_R_V | CST | 5348 | Rabbit |
| PPP1R10(EPR11706)_R_V | Abcam | ab173285 | Rabbit |
| Stat3(D3Z2G)_R_V | CST | 12640 | Rabbit |
| p-TRAP220-MED1(T1457)_R_V | Abcam | ab60950 | Rabbit |
| HIF-2A(D9E3)_R_V | CST | 7096 | Rabbit |
| p-c-Jun(S63)_R_V | CST | 9261 | Rabbit |
| Sin3b_R_V | Abcam | ab101841 | Rabbit |
| p-Rb(S807/811)_R_V | CST | 9308 | Rabbit |
| p-Rb(S780)(C84F6)_R_V | CST | 3590 | Rabbit |
| p-FAK(Y576/577)_R_V | CST | 3281 | Rabbit |
| Ki67_R_V | Vector Laboratories | VP-K451 | Rabbit |
| Integrina4(D2E1)XP_R_V | CST | 8440 | Rabbit |
| Integrina5_R_V | CST | 4705 | Rabbit |
| IntegrinaV_R_V | CST | 4711 | Rabbit |
| Integrinb1(D2E5)_R_V | CST | 9699 | Rabbit |
| Integrinb3(D7X3P)XP_R_V | CST | 13166 | Rabbit |
| Integrinb4_R_V | CST | 4707 | Rabbit |
| PI3Kp85_R_V | CST | 4292 | Rabbit |
| AMPKa(23A3)_R_V | CST | 2603 | Rabbit |
| PI3Kp110a(C73F8)_R_V | CST | 4249 | Rabbit |
| ERa(SP1)_R_V | Thermo Fisher Scientific | RM-9101-S1 | Rabbit |
| MEK6_R_V | Abcam | ab52937 | Rabbit |
| p21_R_V | CST | 2947 | Rabbit |
| Atg12(D88H11)_R_V | CST | 4180 | Rabbit |
| Beclin-1(D40C5)_R_V | CST | 3495 | Rabbit |
| Caspase-3(Asp175)_R_V | CST | 9661 | Rabbit |
| Beta-Catenin(CT)_R_V | Invitrogen | 71-2700 | Rabbit |
| Atg3_R_V | CST | 3415 | Rabbit |
| Atg7_R_V | CST | 8558 | Rabbit |
| LC3A(D50G8)XP_R_V | CST | 4599 | Rabbit |
| LC3B(D11)XP_R_V | CST | 3868 | Rabbit |
| p-EGFR(Y845)_R_V | CST | 2231 | Rabbit |
| p-EGFR(Y992)_R_V | CST | 2235 | Rabbit |
| ErbB2/HER2(Y1248)_R_V | Imgenex | IMG-90189 | Rabbit |
| p-SHC(2431)(Y317)_R_V | CST | 2431 | Rabbit |
| SRD5A1_R_V | Novusbio | H00006715-D01P | Rabbit |
| FAS_R_V | CST | 3189 | Rabbit |
| HexokinaseII(C64G5)_R_V | CST | 2867 | Rabbit |
| PFKFB3 (C-terminal)_R_V | Abcam | ab135820 | Rabbit |
| RRM2 (EPR11820)_R_V | Abcam | ab172476 | Rabbit |
| p-Stat1(Y701)(D4A7)_R_V | CST | 7649 | Rabbit |
| GLDC_R_V | CST | 12794 | Rabbit |
| GLS1_R_V | Proteintech | 20170-1-AP | Rabbit |
| LDHA_R_V | CST | 2012 | Rabbit |
| PHGDH_R_V | CST | 13428 | Rabbit |
| PKM2_R_V | Bethyl Laboratories | A303-660A-M | Rabbit |
| PKM1/2_R_V | CST | 3190 | Rabbit |
| Bcl-xL_R_V | CST | 2762 | Rabbit |
| E-Cadherin(24E10)_R_V | CST | 3195 | Rabbit |
| ALK(D5F3)XP_R_V | CST | 3633 | Rabbit |
| HER2/c-ErbB2_R_V | Dako | A0485 | Rabbit |
| ALDH_M_V | BD Biosciences | 611194 | Mouse |
| p-ATM(S1981)_M_V | CST | 4526 | Mouse |
| Axl_M_V | Abcam | ab54803 | Mouse |
| CD24 Ab2(SN3b)_M_V | Thermo Fisher Scientific | MS-1279-P0 | Mouse |
| Chk2(1C12)_M_V | CST | 3440 | Mouse |
| Cox-2_M_V | BD Biosciences | 610203 | Mouse |
| GATA3_M_V | BD Biosciences | 558686 | Mouse |
| GSK-3a/b_M_V | Santa Cruz Biotechnology | SC-7291 | Mouse |
| HER2/c-ErbB2-P185_M_V | Invitrogen (BioSource) | AHO1011 | Mouse |
| HIF-1a_M_V | BD Biosciences | 610958 | Mouse |
| p-IkappaB-a(S32/36)_M_V | BD Biosciences | 551818 | Mouse |
| S100A7/CBP_M_V | Abnova | H00006278-A01 | Mouse |
| Twist(Twist2C1a)_M_V | Santa Cruz Biotechnology | sc-81417 | Mouse |
| SRC-3(clone 1208/D1)_M_V | BCM-Mab | in house | Mouse |
| c-Myc(clone 1123)_M_V | BCM-Mab | in house | Mouse |
| SRC-2(TIF2)_M_V | BD | 610985 | Mouse |
| SRC-1(clone 1135/H4,1136/H4H6)_M_V | BCM-Mab | in house | Mouse |
| PR(1294)_M_V | Celetta | in house_Celetta | Mouse |
| Rb(4H1)_M_V | CST | 9309 | Mouse |
| p-FAK(Y397)_M_V | BD Biosciences | 611806 | Mouse |
| AR-441_M_V | BCM | NW-Mab70 | Mouse |
| AMPKa(F6)_M_V | CST | 2793 | Mouse |
| Bcl2(Clone-124)_M_V | Dako | M088729-2 | Mouse |
| c-Src(B-12)_M_V | Santa Cruz Biotechnology | sc-8056 | Mouse |
| IGF-IR(3B7)_M_V | Santa Cruz Biotechnology | sc-462 | Mouse |
| Laminin5_M_V | EMD Millipore | MAB19562 | Mouse |
| Aromatase-A(Clone 677H7F10)_M_V | BCM-Mab | in house | Mouse |
| AOX1(cloneAO15)_M_V | Sigma-Aldrich | SAB4200562 | Mouse |
| IDH2_M_V | Abcam | ab55271 | Mouse |
| SCD1 (CD.E10)_M_V | Abcam | ab19862 | Mouse |
Host species for the antibodies and validation status are reported. R, rabbit; M, mouse and V, validated.