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. 2017 Feb 21;5:16056. doi: 10.1038/boneres.2016.56

Figure 1.

Figure 1

Voluntary exercise for 2 weeks increased FNDC5, PGC1α, and irisin expression in bone. Five-week-old male wild-type C57BL/6J mice weighing 17–20 g were randomly housed individually in empty cages (control group, n=18 mice) or in cages with a polycarbonate running wheel for voluntary running (exercise group, n=18 mice) for up to 2 weeks. Bone marrow was removed from bone tissues before qRT-PCR and western blot analysis. (a) qRT-PCR analysis of FNDC5 and PGC1-α mRNA expression in exercised and control bone tissue (*P<0.05, vs control). (b, c) Western blot analysis of FNDC5 and irisin protein expression in (b) exercised and control bone tissue and in (c) exercised and control articular cartilage (**P<0.01, vs control). (d) qRT-PCR analysis of osteogenic markers osterix (OSX), BSP, and osteocalcin (OCN) mRNA in bones of control and exercise group. (*P<0.05, vs control). (e) Representative immunohistochemistry with anti-irisin antibody reveals immunostaining in growth plate, trabecular bone, cortical bone, articular cartilage, and bone–muscle interface of control and exercised bones.