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. Author manuscript; available in PMC: 2017 Dec 26.
Published in final edited form as: Nat Chem Biol. 2017 Jun 26;13(9):1045–1052. doi: 10.1038/nchembio.2417

Figure 3. Subcellularly localized TrxRFP1.

Figure 3

(a,b) Co-localization of nuclear (a) and mitochondrial (b) TrxRFP1 with a nuclear stain DAPI and a mitochondrial stain MitoTracker Green, respectively (Scale bar = 20 µm). (c,d) Fluorescence responses of nuclear (red) and mitochondrial (blue) TrxRFP1 in HEK 293T to various concentrations of H2O2 (c) or auranofin (d), suggesting that TrxRFP1 can selectively sense the subcellular redox changes of Trx in live cells. Fluorescence responses of nuclear (cyan) and mitochondrial (magenta) rxRFP1.1 are also shown as the controls. Data are represented as mean and s.d. of three independent experiments.