FIG 1 .

Neutralizing antibody titers to the chimeric rDENV4/3-M14 virus and parental DENV3 and DENV4 viruses 3 and 18 months post-primary DENV3 infection. (A) Ribbon diagram of the DENV E trimer (domains I, II, and III in red, yellow, and blue, respectively), showing amino acid residues within the 5J7 epitope spanning the A, B, and B′ monomers (purple and teal spheres); the residues represented by the teal spheres were transplanted into a DENV4 backbone, creating the rDENV4/3-M14 virus. (B) As in panel A, but the residues represented by the teal spheres were transplanted into a DENV4 backbone to create the rDENV4/3-M16 virus. (C and D) The raw antibody titration data were fitted with a four-parameter sigmoidal dose-response curve to estimate the 50% neutralizing antibody titer (NT₅₀) to the rDENV4/3-M14 and parental viruses DENV3 and DENV4 in plasma samples 3 and 18 months postinfection, respectively. (E) Geometric mean of the NT₅₀ values to the rDENV4/3-M14 and parental viruses of DENV3 and DENV4 in plasma samples 3 and 18 months postinfection. Data are representative of two independent experiments processed in duplicate for each plasma sample. The NT₅₀ values were compared by two-way ANOVA (n = 30). ***, P < 0.001; ****, P < 0.0001.