FIG 5 .

Emergence of emm89 in ABCs. Notes inserted into the graph describe the numbers of isolates sequenced and relevant pipeline results. The data are consistent with previous observations (4, 5) associating the emergence of emm89 with the appearance of the acapsular clade 3 strain containing the upregulated nga operon promoter Pnga3 and a different tee gene (36). According to our data, the clade 3 strain is T serotype 13 (corresponding to the tee89 gene) and associated with the decline of the T serotype 11 (corresponding to the tee11 gene) clade 1 emm89 strain (5) that expressed capsule and contained the less active nga promoter Pnga1. Although only two T11 (tee11) isolates (recovered in 1995 and 2000) and one T13 (tee89) isolate (recovered in 2000) were sequenced in this study prior to 2015, where all 185 isolates were sequenced, our data are entirely consistent with the previous reports and publicly available WGS data that include a large number (870) of these isolates recovered from 1995 to 2013 (4, 5, 36). Compare Fig. 1 in reference 36, which describes the international emergence of clade 3 emm89 to the data presented here.