FIG 1 .

Schematic representation of the FISH-flow assay alongside that of the QVOA. (A) QVOA. The steps involve the preparation of resting CD4 T cells from HIV-infected individuals, serial dilution and reactivation (day 1), expansion of the reactivated virus (day 2), and subsequent detection of the expanded viruses by quantitation of viral RNA or protein in the supernatant by quantitative RT (qRT)-PCR or by p24 enzyme-linked immunosorbent assay (ELISA), respectively. (B) FISH-flow assay. The steps involve the use of PBMCs directly or after reactivation to score for cells expressing HIV-1 RNA and Gag protein, as well as for cell surface markers (the example shown is for T cell subset markers—shown with antibody-labeled surface markers). FACS, fluorescence-activated cell sorting.