Table 2.
Physical characteristics of uncoated and coated CS-NPs before and after lyophilization without using cryoprotectant (mean ± SD, n = 3).
| Parameters | Before lyophilization |
After lyophilization |
||||
|---|---|---|---|---|---|---|
| Uncoated CS-NPs | HA-coated CS-NPs | ALG-coated CS-NPs | Uncoated CS-NPs (F1) | HA-coated CS-NPs (F2) | ALG-coated CS-NPs (F3) | |
| Polydispersity index | 0.356 ± 0.055 | 0.123 ± 0.015 | 0.566 ± 0.038 | 0.503 ± 0.056 | 0.351 ± 0.134 | 0.769 ± 0.162 |
| Particle size (nm) | 298.11 ± 20.15 | 390.84 ± 27.28 | 843.42 ± 51.74 | 346.33 ± 13.16 | 398.62 ± 13.98 | 925.36 ± 57.89 |
| Zeta potential (mV) | +34.3 ± 5.2 | −36.1 ± 5.4 | −64.2 ± 8.2 | +35.5 ± 4.5 | −34.7 ± 3.9 | −62.3 ± 5.8 |
| Aggregation* | – | – | – | +++ | + | ++ |
*
Aggregation: “+” = minimum, “++” = medium and “+++” = maximum.