Figure 1.

Detection of wild-type and variant WWOX mRNAs using nested reverse transcription-polymerase chain reaction. (A) The wild-type WWOX and variant amplicons migrated as 1,288 and 600 bp bands, respectively. The variant WWOX amplicon was detected in the KMM1, KMS11, KMS18 and RPMI8226 cell lines, and RPMI8226 cells expressed both forms. A weak band was detected in KMM1 and KMS18 cells, in which the WWOX promoter was methylated. (B) Detection of wild-type and variant WWOX amplicons in the plasma cells of patients with MM. WWOX, WW domain containing oxidoreductase; bp, base pairs; MM, multiple myeloma.