Figure 5.

Influence of substrate volume and time required for enzyme-substrate incubation on RLU values. Detection conditions: a standard positive sample (50 ng/ml) was used to evaluate the effect; room temperature and 1 µl of Streptavidin-coated MPs; the HRP-labeled antibody dilution ratio is 1:15,000; the biotinylated antibody dilution ratio is 1:18,000. The six plots correspond to a series of durations required for enzyme-substrate incubation (10,20,30,40,50 and 60 min). RLU: *P<0.05. RLU, relative light unit; MPs, magnetic particles.