Figure 3.

Downregulation of LRIG2 in glioma cell lines inhibited tube formation and cell migration of HUVECs. U87 and U251 cell lines were transfected with si-LRIG2 or si-NC. (A) HUVECs angiogenesis induced by coculture with glioma cells was assessed by using transwell co-cultured system (0.4 µm pore size). (B) HUVECs migration induced by coculture with glioma cells was assessed by using transwell co-cultured system (8 µm pore size). (C) The number of formed tubular structures were calculated. (D) The migrated cells were counted. **P<0.01 vs. the si-NC group. Results are representative of three different experiments. The scale bar is 100 µm. si-LRIG2, LRIG2 siRNA; si-NC, negative control siRNA; HUVECs, human umbilical vein endothelial cells.