Figure 5.

U87 and U251 cell lines were transfected with si-LRIG2 or si-NC, then treated with 100 ng/ml EGF or DDH₂O for 24 h. (A) HUVECs angiogenesis was assessed by using transwell co-cultured system (0.4 µm pore size). (B) HUVECs migration was assessed by using transwell co-cultured system (8 µm pore size). Then the number of (C) formed tubular structures and (D) the migrated cells was calculated. **P<0.01 vs. the si-NC + DDH₂O group. ^##P<0.01 vs. the si-LRIG2 + DDH₂O group. Results are representative of three different experiments. The scale bar is 100 µm. LRIG2, leucine-rich repeats and immunoglobulin-like domains 2; EGF, epidermal growth factor; HUVECs, human umbilical vein endothelial cells; si-LRIG2, LRIG2 siRNA; si-NC, negative control siRNA.