Figure 6.

U87 and U251 cell lines were transfected with si-LRIG2 or si-NC, then treated with 100 ng/ml EGF or DDH₂O for 24 h. (A) The protein expression level of EGFR, p-EGFR and VEGF-A were assessed by western blot assay. (B) The densitometry data of western blotting. **P<0.01 vs. the si-NC + DDH₂O group. ^##P<0.01 vs. the si-LRIG2 + DDH₂O group. Results are representative of three different experiments. LRIG2, leucine-rich repeats and immunoglobulin-like domains 2; EGFR, epidermal growth factor receptor; VEGF-A, vascular endothelial growth factor A; si-LRIG2, LRIG2 siRNA; si-NC, negative control siRNA.