Table 1.
Strains and plasmids used in this study
| Strains and plasmids | Gene types and characteristics | Source |
|---|---|---|
| Strains | ||
| E. coli MG 1655 | F-φ80 lac ZΔM15 Δ(lacZYA-arg F) U169 endA1 recA1 hsdR17(rk − ,mk +) supE44λ- thi − 1 gyrA96 relA1 phoA | Transgene |
| E. coli NK-C1 | MG1655 contains pLsrACDB | This study |
| E. coli NK-C2 | MG1655 contains pLsrACDBK | This study |
| E. coli NK-C3 | MG1655 contains pLsrACDBFG | This study |
| E. coli NK-C4 | MG1655 contains pLsrACDBFGK | This study |
| E. coli SU1 | MG1655 contains pLuxS | This study |
| E. coli SU2 | MG1655 contains pLuxS-Mtn | This study |
| Plasmids | ||
| pTrcHisB | Cloning vector Apr | |
| pLsrACDB | pTrcHisB derivative, containing lsrACDB | This study |
| pLsrACDBFG | pTrcHisB derivative, containing lsrACDB, and lsrFG | This study |
| pLsrACDBK | pTrcHisB derivative, containing lsrACDB and lsrK | This study |
| plsrACDBFGK | pTrcHisB derivative, containing lsrACDB,lsrFG,lsrK | This study |
| pLuxS | pTrcHisB derivative, containing luxS | This study |
| pLuxS-Mtn pLGFP |
pTrcHisB derivative, containing luxS and mtn
pTrcHisB derivative, containing lsr promoter and gfp |
This study This study |