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. 2017 Sep 19;10:64. doi: 10.1186/s13048-017-0354-z

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Early follicular culture in serum free conditions. Each individual follicle was observed and photographed every second day. Two perpendicular diameters of follicles were measured. The mean of two diameters was calculated and considered as the reference diameter of the follicle. Diameters of oocytes were taken in the same fashion. The first day of culture was considered day 0. a. Early follicles (50–150 μm) were mechanically isolated from 8-day-old mice and co-cultured with AOECs (n = 32), Matrigel (n = 27), or gel (n = 24) in serum free media for 14 days. b. Early follicles cultured on AOECs versus gelatin on days 2, 6 and 14. Growth and proliferation seen in AOEC group versus progressive degeneration and eventual oocyte (oo) extrusion in gelatin group. c. Follicular growth in primary follicles (<100 μm). d. Follicular growth of secondary follicles (>100 μm)