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. 2017 Sep 19;10:64. doi: 10.1186/s13048-017-0354-z

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — a. Oocyte growth and estradiol concentrations. a Oocyte growth of oocytes < 45 μm b. Oocytes growth of oocytes >45 μm c. Follicular estradiol secretion. Due to undetectable levels of Estradiol in primary follicles and from individual follicles, we cultured 5 secondary follicles (100–150 μm) in each well