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. 2017 Sep 19;10:43. doi: 10.1186/s13072-017-0151-3

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Asx co-fractionates and co-immunoprecipitates with Trx in embryo nuclear extracts. a Schematic of Bio-Rex-70 fractionation of embryo nuclear extracts and coupled co-immunoprecipitation–western blot analysis. b Western blot analysis of Bio-Rex 70 fractions with anti-Asx (upper panel), anti-Trx (middle panel) and anti-E(z) (lower panel). c Coupled anti-Asx immunoprecipitation and anti-Trx western blot analysis of the BR70-0.85 fraction, showing Asx association with Trx. d Coupled anti-Asx immunoprecipitation and anti-E(z) western blot analysis of the BR70-0.1 fraction, showing lack of Asx association with E(z) in the enriched E(z) fraction. e Coupled anti-Asx immunoprecipitation and anti-E(z) western blot analysis of the BR70-0.85 fraction, showing lack of Asx association with E(z) in the enriched Trx fraction