Fig. 5.

ASIC2 activates the calcineurin/NFAT1 pathway under acidosis. Cells were cultured at pH 7.4 and pH 6.5 for 24 h. The whole-cell extracts and nuclear extracts from (a) SW480 and (b) SW620 cells were used to analyze ASIC2 or NFAT1 by western blotting. Cell invasion ability was measured in the presence or absence of Cyclosporine A at the indicated concentration. c Cyclosporine A significantly inhibited ASIC2-induced invasion of SW480 cells under an acidic environment. d Cyclosporine A significantly inhibited acid-induced invasion of SW620 cells. *p < 0.05, **p < 0.01