Table 1.
Characteristics of the HRM qPCR assay performances
| Parameter | PCR assay | ||
|---|---|---|---|
| HRM qPCR for ITS1 | HRM qPCR for 18S | HRM qPCR for cytb | |
| Efficiency (%) | 90 | 103 | 134 |
| Slope | -3.61 | -3.23 | -2.70 |
| y-intercept | 35.15 | 34.88 | 40.12 |
| R2 value | 0.9976 | 0.9962 | 0.9896 |
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