Table 2.
Detection of S. lupi eggs in fecal samples from dogs by five different assays
| Fecal sample code number | HRM qPCR for ITS1 (estimated epg) | HRM qPCR for 18S (estimated epg) | HRM qPCR for cytb (estimated epg) | Semi-nested PCR for cox1 | Fecal flotation (estimated epg) |
|---|---|---|---|---|---|
| A-01F | + (13.1 ± 9.0) | + (2.2 ± 0.1) | – | – | + (120) |
| C-01F | – | – | – | – | – |
| D-01F | + (106.8 ± 45.5) | + (28.5 ± 8.2) | + (5.7 ± 1.8) | + | + (10) |
| E-01F | + (0.7 ± 0.1) | + (0.03 ± 0.001) | – | – | + (12) |
| F-01F | – | – | – | – | – |
| G-01F | – | – | – | – | – |
| I-01F | + (7.8 ± 1.0) | + (0.3 ± 0.04) | – | – | – |
| J-01F | + (0.7 ± 0.1) | – | – | – | – |
| K-01F | + (0.5 ± 0.1) | – | – | – | – |
| L-01F | – | – | – | – | – |
| M-01F | + (0.7 ± 0.1) | – | – | – | – |
| N-01F | + (2.3 ± 0.5) | + (0.3 ± 0.1) | – | – | + (1) |
| O-01F | + (41.4 ± 9.8) | + (17.0 ± 3.1) | + (0.1 ± 0.06) | – | + (160) |
| P-01F | – | – | – | – | – |
| Q-01F | – | – | – | – | – |
| S-01F | – | – | – | – | – |
| T-01F | + (8.6 ± 2.2) | + (0.97 ± 1.1) | – | – | – |
| U-01F | + (12.3 ± 1.5) | + (4.4 ± 0.1) | – | – | – |
| Total | 11 | 8 | 2 | 1 | 5 |