Table 1.
Primers used for RT-PCR
| Heading | Forward Sequence (5´➔3′) | Reverse Sequence (3´➔ 5′) | Accession Nr. | Efficiency |
|---|---|---|---|---|
| Human | ||||
| TNF-α | ATGGGCTACAGGCTTGTCACTC | CTCTTCTGCCTGCTGCACTTTG | NM_ 000594 | 1.80 |
| LT-α | TCCGTGTTTGCTCTCCAGAGCA | ACACCTTCAGCTGCCCAGACTG | NM_ 000595 | 1.8 |
| LT-β | CGTCAGAAACGCCTGTTCCTTC | GGTTTCAGAAGCTGCCAGAGGA | NM_ 000595 | 1.97 |
| Fas-L | CTGTGTGCATCTGGCTGGTAGA | GGTTCTGGTTGCCTTGGTAGGA | NM_000639 | 1.98 |
| TRAIL | AGCTGCTACTCTCTGAGGACCT | TGGCAACTCCGTCAGCTCGTTA | NM_003810 | 1.73 |
| TWEAK | TGGAGCTGTTGATTCTGGCTTCC | AAAACACGGGCTCGAAGAGCGA | NM_003809 | 2.14 |
| 4-1BBL | TGGAAATCGGCAGCTACAGCCA | TCTTCCTCACGCTCCGTTTCTC | NM_003811 | 2.08 |
| OX40-L | TGATGACTGAGTTGTTCTGCACC | CCTACATCTGCCTGCACTTCTC | NM_003326 | 2.10 |
| APP | CCTTCTCGTTCCTGACAAGTGC | GGCAGCAACATGCCGTAGTCAT | NM_000484 | 2.15 |
| Mouse | ||||
| TNF-α | GGTGCCTATGTCTCAGCCTCTT | GCCATAGAACTGATGAGAGGGAG | NM_013693 | 2.20 |
| LT-α | AGCCCATCCACTCCCTCAGAAG | TGCTCTCCAGAGCAGTGAGTTC | NM_010735 | 1.94 |
| LT-β | CCTGTTGTTGGCAGTGCCTATC | GACGGTTTGCTGTCATCCAGTC | NM_008518 | 2.22 |
| Fas-L | GCCACACTCCTCGGCTCTTTTT | GAAGGAACTGGCAGAACTCCGT | NM_010177 | 2.12 |
| TRAIL | TGGAGTCCCAGAAATCCTCA | TCACCAACGAGATGAAGCAG | NM_009425 | 1.95 |
| TWEAK | ACACCGTTCACCAGCAAGTCCA | CTGCGCTACGACCGCCAGATT | NM_013749 | 1.93 |
| 4-1BBL | GCGTTGTGGGTAGAGGAGCAAA | CCAAGTACCTTCTCCAGCATAGG | NM_009404 | 2.06 |
| OX40-L | CTGGTAACTGCTCCTCTGAGTC | GGAAGAAGACGCTAAGGCTGGT | NM_009452 | 1.81 |
| APP | GCCAAGACATCGTCGGAGTAGT | TCCGTGTGATCTACGAGCGCAT | NM_177310 | 2.11 |