Table 1.
Effect of organic solvents and surfactants on the enzyme activity of McCut
| Solvent | Specific activity (U/mg) | Relative activity (%) |
|---|---|---|
| Organic solvent (30%) | ||
| Control | 1115.4 ± 11.5 | 100 |
| Methanol | 891.6 ± 4.6a | 79.9 |
| Ethanol | 811.6 ± 43.9a | 72.8 |
| Isopropanol | 908.8 ± 40.4a | 81.5 |
| Butanol | 656.4 ± 16.2a | 58.8 |
| Acetic acid | 649.1 ± 17.3a | 58.2 |
| Butyric acid | 398.3 ± 18.5a | 35.7 |
| n-Hexane | 1090.1 ± 35.8 | 97.7 |
| Cyclohexane | 1426.6 ± 32.8a | 127.9 |
| n-Heptane | 1465.0 ± 11.6a | 131.3 |
| Octane | 1601.1 ± 8.2a | 143.5 |
| Isooctane | 1089.3 ± 39.3 | 97.7 |
| Acetone | 983.1 ± 6.9a | 88.1 |
| Dimethyl sulfoxide | 902.3 ± 33.5a | 80.9 |
| Acetonitrile | 1038.1 ± 7.7a | 93.1 |
| Surfactant (5%) | ||
| Tween 20 | 1316.1 ± 39.3a | 118.0 |
| Tween 40 | 1557.9 ± 55.4a | 139.7 |
| Tween 60 | 1555.7 ± 81.4a | 139.5 |
| Tween 80 | 1582.4 ± 20.8a | 141.9 |
| Triton X-100 | 2120.9 ± 40.8a | 190.1 |
| SDS | 1468.6 ± 44.6a | 131.7 |
The enzyme was incubated with various organic solvents and surfactants at 50 °C for 1 h in 50 mM Tris–HCl pH 8.0, and the residual activity was measured according to the standard method. All data are mean values ± standard deviations of triplicate measurements
aThe difference is significant compared with the control (P < 0.05)