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. Author manuscript; available in PMC: 2017 Sep 20.
Published in final edited form as: Cancer Res. 2008 Jan 1;68(1):172–180. doi: 10.1158/0008-5472.CAN-07-2678

Figure 5.

Figure 5

Schematic diagram of the putative human chk-α promoter region-firefly Luc-based reporter assay constructs. Top, the 2.3-kb region of the human chk-α gene, which is immediately upstream of the translation start site (+1). Putative HRE binding sites (core sequence RCGTG) are labeled HRE 1 to HRE 6 and represented by vertical orange bars. Bottom, P1, entire 2.3-kb Luc reporter construct; P2 to P7, Luc-reported constructs of truncated regions of this 2.3-kb sequence. All constructs were contained in the pGL4-basic vector and tested for endogenous HIF-1–mediated hypoxia-induced Luc expression in transient transfections of wild-type PC-3 and PC-3-5HRE-EGFP cells.