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. Author manuscript; available in PMC: 2017 Dec 7.
Published in final edited form as: Sci Transl Med. 2017 Jun 7;9(393):eaal1669. doi: 10.1126/scitranslmed.aal1669

Fig. 1.

Fig. 1

Crossing Abcc6 to Enpp1 or Nt5e mutant mice reveals genetic interaction.

Abcc6 mutant mice were crossed to Enpp1 or Nt5e mutant mice to generate all possible genetic allele combinations. (A and C) Micro-CT scans of the muzzle to evaluate the extent of vibrissae fibrous capsule calcification were obtained at 15 weeks of age. Representative coronal z-stacked images of the mouse muzzle with the nasal bones and sinuses midline (indicated by white asterisk) and the pathological calcification seen as radiodense lesions (indicated by the yellow arrow) in the surrounding soft tissue. (B and D) Quantification of ectopic calcification from micro-CT images. A two-way ANOVA with Tukey’s honest significance difference post hoc analysis was performed. Two-way ANOVA: (B) Abcc6 effect, P = 2.2 × 10−16; Enpp1 effect, P = 2.2 × 10−16; interaction effect, P = 2.2 × 10−16; (D) Abcc6 effect, P = 2.2 × 10−16; Nt5e effect, P = 1.2 × 10−4; interaction effect, P = 1.1 × 10−3. P values of post hoc comparisons are indicated in the figure.