Fig. 4.

Evidence that both local and systemic defects in ATP metabolism are needed to promote PXE-associated ectopic calcification in mice.

(A) Micro-CT scans of the muzzle demonstrating ectopic calcification at 20 weeks of age upon deletion of Abcc6 in both the liver and local Wnt1⁺ cells in the fibrous capsule, albeit with reduced penetrance compared to constitutive targeting (Fig. 3, A and B). (B) Quantification of ectopic calcification from micro-CT images. (C) Visualization and confirmation of Cre-targeted tissues and cell types using the Rosa^mTmG reporter mouse line. Representative images are shown.