Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
| Method | Hanging drop |
| Plate type | 24-well |
| Temperature (K) | 290 |
| Final DNA concentration (mM) | 1 |
| Buffer composition of DNA solution | 60 mM sodium cacodylate pH 7.0, 17 mM MgCl2, 2 mM spermine |
| Composition of reservoir solution | 50%(v/v) MPD |
| Volume and ratio of drop | 3 µl (1:1:1 DNA:DAPI:reservoir) |
| Volume of reservoir (µl) | 550 |
