Table 3.
Identification of major protein bands on SDS PAGE. The observed mass was estimated by SDS PAGE.
| Protein Identification/ SwissProt Accession |
Protein Mass | Tryptic Peptides Identified |
Mowse Score | |
|---|---|---|---|---|
| Observed | Reported | |||
| 1) Transferrin P02787 | 80,000 | 79,600 | DLLFK | 337 |
| DGAFDVAFVK | ||||
| SASDLTWDNIK | ||||
| MYGYEYVTAIR | ||||
| 2) α1-Proteinase Inhibitor P01009 | 50,000 | 50,300 | IVDLVK | 173 |
| SPLFMGK | ||||
| SASLHLPK | ||||
| VFSNGADLSGVTEEAPLK | ||||
| 3) Leucine-rich α2-glycoprotein P02750 | 48,000 | 50,000 | VLDLTR | 128 |
| GPLQLER | ||||
| GQTLLAVAK | ||||
| VAAGAFQGLR | ||||
| 4) α1-Acid glycoprotein P02763 | 40,000 | 40,000 | SDVVYTDWK | 108 |
| EQLGEFTEALDCLR | ||||
| YVGGQEHFAHLLILR | ||||
| 5) Zinc-α2-glycoprotein P25311 | 39,000 | 38,500 | SSGAFWK | 495 |
| IDVHWTR | ||||
| AGEVQEPELR | ||||
| AYLEEECPATLR | ||||
| 6) α1-Microglobulin P02760 | 25,000 | 27,000 | VVAQGVGIP | 33 |
*
Mowse scores > 46 indicate identity or extensive homology with the specified protein (p < 0.05). For α1-microglobulin, mass spectrometric data was combined with electrophoretic mobility to confirm the identification.