. Author manuscript; available in PMC: 2018 Jan 1.

Published in final edited form as: Biol Chem. 2017 Jan 1;398(1):101–112. doi: 10.1515/hsz-2016-0141

Table 2.

Kinetic parameters^a for the hydrolysis of dipeptidyl-2-naphthylamide substrates catalyzed by the Arabidopsis thaliana Nudix-DPP III (AtND).

Substrate	K_m (µM)	k_cat (min⁻¹)	k_cat/K_m (M⁻¹ s⁻¹)
Ala-Arg-2NA	5.0 ± 0.3	2.49 ± 0.21	8300.0
Pro-Arg-2NA	40.85 ± 12.09	2.23 ± 0.3	908.2
Arg-Arg-2NA	3.25 ± 1.12	0.120 ± 0.001	615.4
Ala-Ala-2NA	240.2 ± 69.4	2.28 ± 0.41	158.2

The kinetic parameters were determined from the initial reaction rates at 25 °C, as described in Materials and methodss. K_m and k_cat values are presented as mean ± SD