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. Author manuscript; available in PMC: 2017 Dec 21.
Published in final edited form as: Nature. 2017 Jun 21;547(7662):236–240. doi: 10.1038/nature22822

Extended Data Figure 7. Measuring Shogushin 1, Lamin B Receptor, H3K9me3 peptide affinity, and the effect of Shogushin peptide binding on oligomerization.

Extended Data Figure 7

a, b, Fluorescence anisotropy plots showing the Kd measurements (values in μM next to symbols for WT vs. CSDm) for LBR and Sgo1 peptide binding to WT HP1α and the I163A CSD mutant (CSDm) which can no longer form dimers. c, Comparative AUC runs of ~50 μM nPhos HP1α with and without 100 μM Shogushin peptide or LBR peptide. d, Fluorescence anisotropy plots with a 15mer trimethylated H3K9 peptide showing the relevant HP1 isoforms can bind the nucleosome tail.