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PDGF-AA does not protect RGCs in culture. (A) RGCs isolated by immunopanning were stained with antibodies against acetylated tubulin and MAP2. Scale bar: 50 μm. (B) Western blot analysis of native retina, mixed retinal cultures, and isolated RGCs. (C) Survival of RGCs in culture in the presence of PDGF-AA (50 ng/mL), BDNF (50 ng/mL), and CNTF (50 ng/mL) or PDGF-AA, BDNF, and CNTF together. **P < 0.01; ***P < 0.001; NS, not statistically significant.
