Table 1.

Differentiating the two serovars of Chlamydia using an in-house multiplex real-time PCR (first nine weeks of the study); serovar E was inoculated in 7 animals (STI arm) at week 1 followed by inoculation of serovar LGV-L₂ at week 4. The first SHIV_163p3 challenge (red arrow) was in week 5; at week 8, both serovars were inoculated in the animals. Throughout the study, the animals received a boost of Chlamydia (both serovars) every 4 weeks, and up to 20 SHIV challenges two times a week (red arrows).

	E		LGV				SHIV E+LGV
Animal #	Week 1	Week 3	Week 4	Week 5	Week 6	Week 7	Week 8	Week 9
1	ND	E	E	E	E*	M	M	E
2	ND	E	E	M	M	M*	M	M
3	ND	E	N	N	N*	E	N	E
4	ND	E	E	M	E	N	E	E
5	ND	E	E	E	E	E	E	E
6	ND	E	E	M	LGV	E	E	E
7	ND	E	E	E	E	N	E	E

^*=animal SHIV-infected;

ND=not determined; E=serovar E; LGV=serovar LGV-L2; M=mixed population; N=negative result (no Chlamydia detected). Animal #4 was infected after 14 SHIV challenges (this table shows data only up to 9 viral challenges).