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. Author manuscript; available in PMC: 2017 Sep 20.
Published in final edited form as: Sci Transl Med. 2017 Aug 30;9(405):eaai8710. doi: 10.1126/scitranslmed.aai8710

Figure 5. Dual editing of endothelial and perivascular cells promotes incorporation of regenerative human hepatocytes in the liver.

Figure 5

(A) Schema depicting the strategy to transplant human hepatocytes into NCG mice.

(B) Distribution of grafted GFP+ human hepatocytes in the recipient mouse liver after indicated treatments. GFP was co-stained with cholesterol 7 alpha-hydroxylase (CYP7A1) in the liver sections from recipient injured mice.

(C, D) Cell apoptosis in NCG mice with indicated treatments and transplanted with hepatocytes. TUNEL was co-stained with HNF4 and VE-cadherin. n = 5 mice per group.

(E–G) Hepatic pathology and quantity of serum human albumin and bilirubin in recipient mice after BDL. n = 5 mice per group.

(H) Dual editing of vascular and perivascular niches by endothelial Hgf gene delivery and NOX4 inhibition facilitates parenchymal cell engraftment and promotes liver repair. Statistical analysis in Figure 5 was carried out with one-way ANOVA followed by Tukey’s test as post hoc analysis. Scale bars, 50 um.