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Tag-less CFA/I/II/IV MEFA protein expression and characterization. Left: SDS-PAGE Coomassie blue staining to show purity of refolded CFA/I/II/IV MEFA proteins. 9175, 6xHis-tagged CFA/I/II/IV MEFA (~17 kDa); 9472, tag-less CFA/I/II/IV MEFA (~15 kDa). Middle: Western blot with mouse anti-CFA/I antiserum to detect the refolded 6xHis-tagged or the tag-less CFA/I/II/IV MEFA protein. Right: Mass spectrophotometer to show the mass of the refolded tag-less CFA/I/II/IV MEFA protein.
