Figure 2.
ARC overexpression reduces amyloid-induced β-cell apoptosis and β-cell loss without altering amyloid formation. Nontransgenic (NT) and hIAPP transgenic (TG) islets were transduced with AdV control (AdV-GFP) or AdV-hARC and then cultured for 144 h in 16.7 mmol/L glucose. Islet protein was collected to determine efficiency of overexpression (A). Islets were then fixed for histological determination of rates of β-cell apoptosis (B), β-cell area (C), and amyloid severity (D). n = 4–5. *P < 0.05, **P < 0.01, ***P < 0.001.
