Figure 1.

Schematic overview of design of vaccine antigens in this study. (A) LS_PB10 constructs with four different linkers and corresponding sample identification code; the sequence of PB10 peptide is “NQEDAEAITHLF” (B) Hypothetical 3‐D structure of LS_PB10 and LS_PB10_CL constructs (CL indicates formaldehyde crosslinking). The in silico model was manually constructed by combining the 60‐fold lumazine synthase structure from B. anthracis (PDBID: 4V7G), with the linear PB10 epitope from R. communis (PDBID: 3SRP), enjoined by an extended glycine‐serine linker. The PB10 epitope is shown in dirty violet color. The possible covalent bonds induced by formaldehyde crosslinking are shown in red color. The hypothetical structures were modeled using the program PyMOL v 1.7 (https://www.pymol.org/)