Table 1.
Size Analysis of LS and LS_PB10 Capsid Constructs
| Sample | Hydrodynamic Diameter (nm) | Polydispersity | V R(mL) | Measured MW(MDa) | Theoretical MW(MDa) |
|---|---|---|---|---|---|
| LS | 15.5 ± 0.2 | 0.032 | 13.4 ± 0.1 | 0.9510.02 | 0.97 |
| LS_PB10_12 | 18.2 ± 0.2 | 0.067 | 12.5 ±0.1 | 1.0810.01 | 1.09 |
| LS_PB10_12 CL | 18.2 ± 0.5 | 0.071 | 12.8 ± 0.1 | 1.12 + 0.01 | NA |
| LS_PB10_34 | 17.6 ± 0.0 | 0.045 | 12.5 ± 0.2 | 1.07 + 0.01 | 1.08 |
| LS_PB10_34 CL | 17.6 ± 0.2 | 0.034 | 12.8 ± 0.0 | 1.11 + 0.01 | NA |
| LS_PB10_56 | 18.8 ± 0.3 | 0.031 | 12.410.1 | 1.0710.01 | 1.11 |
| LS_PB10_56 CL | 19.1 ±0.1 | 0.007 | 12.610.0 | 1.18 + 0.01 | NA |
| LS_PB10_78 | 18.4 ± 0.2 | 0.071 | 12.310.1 | 1.0910.01 | 1.11 |
| LS_PB10_78 CL | 18.6 ± 0.1 | 0.062 | 12.410.0 | 1.16 + 0.01 | NA |
Hydrodynamic diameter and polydispersity was determined by DLS, elution volume (V R) by SEC, and absolute molecular weight by MALS. Error values are standard deviations (N = 3). See Figure 1 for description of samples.
Theoretical MW was calculated by multiplying the average molecular weight of each monomer with sixty. NA indicates that it is not possible to predict the theoretical MW for crosslinked constructs.