Figure 5.

Use of the Buffer Mismatch Model (BMM) in Sedfit allows for correction of nonoptimal sedimentation velocity conditions. (A) A typical c(s) interference data set from a Brpt1.5 experiment in metal‐free MOPS buffer. Circles show every 10^th data point of every 3^rd scan from the first half of the loaded data set, superimposed by lines showing the modeled fit. (B) The same selection of data from the Brpt5.5 sample with 4 mM CuSO₄ is an example of a case where the BMM rescues a condition that would not be accessible using traditional c(s) methods. Note the distinct differences in the shape of the boundaries and the abnormal baseline caused by co‐sedimentation of the Cu²⁺ ions and mismatch with the buffer blank in the reference sector.