Figure 7.

Hydrodynamic modeling of Brpt1.5 and Brpt5.5. Brpt1.5 crystal structures7 were used for hydrodynamic modeling of monomer and dimer species. (A) A low‐resolution bead model of the Brpt1.5 monomer is created from the coordinates of each crystal structure and combined into an ensemble used to predict the average sedimentation coefficient and frictional ratio. (B) Structures of the Zn²⁺‐induced Brpt1.5 dimer7 were used to create a bead model to estimate the sedimentation parameters of a homogenous Brpt1.5 dimer population. (C) Since no structure of Brpt5.5 has currently been solved, various Brpt1.5 structures7, 8 were combined to create an ensemble of homology models for the longer construct, yielding predicted sedimentation parameters that were similar to experimental values.